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Profile: Rashim Singh

Rashim Singh
Rashim Singh, Ph.D.
Research Assistant Professor of Pharmaceutics

Health 2
University of Houston College of Pharmacy
4849 Calhoun Road, Room N/A
Houston, TX 77204-5037

rsingh3@central.uh.edu
Office: N/A

Education

Ph.D. in Pharmaceutics, College of Pharmacy, University of Houston, Houston, TX

Masters in Pharmaceutical Technology (Formulation), National Institute of Pharmaceutical Education and Research (NIPER), Punjab, India

Bachelor in Pharmacy (B.Pharm), Institute of Technology, Banaras Hindu University, Varanasi, India

Research Interests

  • Pharmacokinetics of flavonoids and herbal formulas
  • Quantitative Structure-dispositions Relationship of Flavonoids
  • Flavonoids-gut microbiome interaction in human gut and brain health
  • Targeted oral delivery of compounds to lower gastrointestinal tract
  • Drug-induced intestinal toxicity and inflammation-mediated gut diseases/disorders

Awards & Honors

  • American Society of Microbiology Science Teaching Fellowship, 2016-17
  • NIH Post-doctoral Training Fellowship under NIGMS Research Supplements to Promote Re-entry into Biomedical and Behavioral Research Careers, 2016-18
  • NIH Pre-doctoral Training Fellowship under Pharmacoinformatics interdisciplinary training program of the Keck Center of Gulf Coast Consortia, 2007-09
  • Phi Beta Delta Honors Society for International Scholars, Membership for Outstanding Achievement, 2006-2010
  • The Rho Chi Pharmacy Honors Society, University of Houston Chapter, 2007-2010
  • Outstanding Chair Award by American Association of Pharmaceutical Scientists (AAPS) Student Chapter, University of Houston, 2008
  • Yoginder Nath Goel & Swaran Lata Goel Academic Achievement Award Endowed Scholarship, University of Houston, 2008
  • Prof. M. L. Schroff Award, Indian Pharmaceutical Congress (IPC), 2001
  • GP NAIR Award, Indian Drug Manufacturers Association (IDMA), 2001
  • University Gold Medal and “Arun & Malvia” Medal, Banaras Hindu University, 2001

Publications

Peer-reviewed Journals
"*" equal contributing authors; "#" corresponding author  

Singh R*, Lu R and Hu M* (2020). Flavonoids Interference in Common Protein Assays: Effect of Position and Degree of Hydroxyl Substitution. Analytical Biochemistry, May 15;597:113644.

Bui D T, Li L, Yin T, Wang X, Gao S, You M, Singh R*, Hu M* (2020). Pharmacokinetic and metabolic profiling of Magnolia officinalis extract key active components against oral cancer using a validated LC-MS/MS method. J Agric Food Chem, 68, 24, 6576–6587

Tu Y, Wang L, Rong Y, Tin T, Gao S, Singh R, and Hu M. Hepatoenteric Recycling: A New Disposition Mechanism for Orally Administered Phenolic Drugs and Phytochemicals (under review).

Yun C, Dashwood W, Kwong L N, Gao S, Yin T, Ling Q, Singh R, Dashwood R H, Hu M. (2018) Accurate quantification of PEG2 in the polyposis in rat colon (Pirc) model by surrogate analyte-based UPLC–MS/MS. J Pharm Biomed Anal,148:42-50.

Meng S, Wu B, Singh R, Yin T, Morrow JK, Zhang S, Hu M. (2012) SULT1A3-mediated regiospecific 7-O-sulfation of flavonoids in Caco-2 cells can be explained by the relevant molecular docking studies. Mol Pharm, 9(4):862-73.

Singh R, Wu B, Tang L and Hu M. (2011) Uridine diphosphate glucuronosyltransferase isoform-dependent regiospecificity of glucuronidation of flavonoids. J Agric Food Chem, 59(13):7452-64.

Singh R, Wu B and Hu M. (2010) Identification of the Position of Mono-O-Glucuronide of Flavones and Flavonols by Analyzing Shift in Online UV Spectrum (λmax) Generated from an Online Diode-arrayed Detector. J Agric Food Chem, 58(17):9384–95

Wu B, Morrow J K, Singh R, Zhang S and Hu M. (2010) Three-dimensional Quantitative Structure-activity Relationship Studies on UGT1A9-mediated 3-O-Glucuronidation of Natural Flavonols Using a Pharmacophore-based Comparative Molecular Field Analysis Model. J Pharmacol Exp Ther, 336(2):403-13.

Tang L, Ye L, Singh R, Wu B, Lv C, Zhao Jie, Liu Z and Hu M. (2010) Use of Glucuronidation Fingerprinting to Describe and Predict mono- and di- Hydroxyflavone Metabolism by Recombinant UGT Isoforms and Human Intestinal and Liver Microsomes. Mol Pharm, 7(3):664–679.

Tang L, Singh R#, Liu Z and Hu M. (2009) Structure and Concentration Changes Affect Characterization of UGT Isoform-Specific Metabolism of Isoflavones. Mol Pharm, 6(5):1466-82. *Equal contribution authors

Xu H, Kulkarni K, Singh R, Yang Z, Wang S, Tam V and Hu M. (2009) Disposition of Naringenin Differs from Apigenin and Is Affected by Compensating Efflux Transporters of Hydrophilic Glucuronides. Mol Pharm, 6(6):1703-15.

Book Chapters and Review Articles

Yang G, Ge S, Singh R, Basu S, Shatzer K, Zen M, Liu J, Tu Y, Zhan C, Wei J, Shi J, Zhu L, Liu Z, Wang Y, Gao S, and Hu M. (2017) Glucuronidation: Driving Factors and Their Impact on Glucuronide Disposition. Drug Metab Rev, 49(2):105-13

Singh R and Hu M. (2011) "Drug Metabolism in Gastrointestinal Tract" in Oral Bioavailability: Basic Principles, Advanced Concepts and Applications, Eds. M. Hu and X. Li. Wiley Press, pg 91-110

Abstracts & Presentations

Invited Presentations

"Triple Recycling of Flavonoids: Interplay of Enzymes, Efflux and Uptake Transporters," AAPS Student Chapter Speaker Event, National Institute of Pharmaceutical Research and Education, India (2019)

"Importance of Research Ethics, Rigor and Reproducibility," AAPS Student Chapter Speaker Event, National Institute of Pharmaceutical Research and Education, India (2019).

Contributed Poster Presentations

"Isoform-Specific Glucuronidation of Five Sub-classes of Flavonoids by Recombinant Human-UGT Isoforms." Rashim Singh, Xing Liu and Ming Hu. International Society for the Study of Xenobiotics, 14th North American Meeting, Puerto Rico (2006).

"Effect of Structure on Hepatic and Intestinal Glucuronidation of Four Subclasses of Flavonoids in Rats." Rashim Singh, Stephen Wang and Ming Hu. Association of Pharmaceutical Scientists Annual Meeting, San Antonio, TX (2006).

"Prediction of Flavonoids Metabolism by UDP-glucuronosyltransferases Enzyme." Rashim Singh, James M. Briggs and Ming Hu. 17th Keck Center Annual Research Conference, League City, TX (2007).

"Effect of Number and Position of Hydroxyl group on the Metabolism of Flavonoids by Expressed Human UGT Isoforms." Rashim Singh and Ming Hu. American Association of Pharmaceutical Sciences Annual Meeting and Exposition, San Diego, CA (2007).

"Effect of Structure on the Transport of Flavonoids in Caco-2 Cell Culture Model." Rashim Singh and Ming Hu. 15th North American Meeting of International Society for the Study of Xenobiotics, San Diego, CA (2008).

"Generation of UGT Isoform-specific Pharmacophores to Predict Metabolism of Flavonoids." Rashim Singh, James Briggs and Ming Hu. 18th Keck Center Annual Research Conference, Houston, TX (2008)

"Correlation between the Rates of Metabolism of Flavonoids by Recombinant Human UGT Isoforms and Caco-2 Cell Culture Model." Rashim Singh and Ming Hu. AAPS Annual Meeting and Exposition, Atlanta, GA (2008).

"Effect of Structural Changes on the Isoform-Specific Glucuronidation of Six Isoflavones by Recombinant Human UGT Isoforms." Lan Tang, Rashim Singh, Ming Hu. 15th North American Meeting of International Society for the Study of Xenobiotics, San Diego , CA (2008).

"Pharmacophore and 3-D QSAR In-silico Modeling of Glucuronidation of Flavonoid by UGT1A8." Rashim Singh, Baojian Wu, Lan Tang, James Briggs and Ming Hu. AAPS Annual Meeting and Exhibition, Los Angeles, CA (2009).

"Prediction of Disposition of Flavonoids in Caco-2 Cells using their Rates of Glucuronidation by Human Recombinant UGT Isoforms." Rashim Singh, Baojian Wu, Lan Tang and Ming Hu. AAPS Annual Meeting and Exhibition, Los Angeles, CA (2009).

"Construction of 3-OH specific models using pharmacophore-based CoMFA to predict glucuronidation of flavonols." Baojian Wu, Rashim Singh, Shuxing Zhang, Ming Hu. AAPS Annual Meeting and Exposition, New Orleans, LA. (2010).

"Efflux Transporters Play Rate-limiting Role in the Excretion of Glucuronides of Flavones but not Flavonols in the Caco-2 Cell Culture Model." Rashim Singh, Baojian Wu, Lan Tang and Ming Hu. AAPS Annual Meeting and Exposition, New Orleans (2010).

"Isoform-specific Regiospecificity and Substrate-selectivity of UGT1A1, UGT1A8 and UGT1A9 in Glucuronidating Flavonoids." Rashim Singh, Lan Tang, Baojian Wu and Ming Hu. AAPS Annual Meeting and Exposition, New Orleans (2010).

"Role of BCRP and MRPs in the Disposition of Galangin Conjugates in Caco-2 and Hela 1A9 MRP3-overexpressed Cell Lines." Rashim Singh, Rong Lu, Taijun Yin, and Ming Hu, American Association of Pharmaceutical Scientists Annual Meeting. San Diego, CA (2017).

"Simultaneous quantification of three key active components of Magnolia officinalis: honokiol, magnolol and 4-O-methylhonokiol by LC-MS/MS method." Dinh Bui, Li Li, Taijun Yin, Rashim Singh, Song Gao, Ming You, Ming Hu. American Association of Pharmaceutical Scientists Annual Meeting. Washington, DC. (2018).

"Intestinal Metabolism and Transport of Dietary Carcinogen PhIP." Rashim Singh, Song Gao, Taijun Yin, and Ming Hu. American Association of Pharmaceutical Scientists Annual Meeting. Washington, DC. (2018).

"Role of Enterohepatic Recycling in Hepatic Disposition of Dietary Carcinogen PhIP." Rashim Singh, Yifan Tu, Terry Yin, Song Gao, Ming Hu*. American Association of Pharmaceutical Scientists Annual Meeting. San Antonio, TX (2019).

"Regional Intestinal Disposition of Desaminotyrosine, An Immunomodulatory Microbial Metabolite of Flavonoids." Rashim Singh, Jin Ke, Taijun Yin, Song Gao, Ming Hu*. International Society for the Study of Xenobiotics, 12th International Meeting. Portland, OR (2019).

"Simultaneous Determination Four Key Active Components of a Chinese Traditional Medicine Zeng sheng ping (Antitumor B) in Mouse Blood and Saliva by LC-MS/MS." Dinh Bui, Song Gao, Taijun Yin, Ming You, Rashim Singh, Ming Hu*. American Association of Pharmaceutical Scientists Annual Meeting. San Antonio, TX (2019).

Memberships

  • International Society of Study of Xenobiotics

  • American Association of Pharmaceutical Scientists

  • American Society of Microbiology