Associate Dean for Research and Graduate Programs
Joseph P. and Shirley Shipman Buckley Endowed Professor of Drug Discovery
Professor of Pharmacology
University of Houston College of Pharmacy
4849 Calhoun Road, Room 5027
Houston, TX 77204-5037
Post-Doctorate – Pharmacology, East Carolina University, Greenville, NC
Our research is focused on understanding the cellular and molecular regulation of the Angiotensin hormone and its receptor expression and the physiological and therapeutic role of these molecules (especially AT2 receptor) in improving renal-cardiovascular function, lowering blood pressure and reducing body weight and inflammation. These investigations utilize molecular/biochemical, cellular and physiological/metabolic approaches, including renal function and blood pressure measurements in various obesity animal models.
Angiotension binds to AT1 and AT2 receptors. Compared to the AT2 receptors, the AT1 receptor are expressed in greater density and mediate most of the known actions of Angiotensin II, such as vasoconstriction, anti-natriuresis, and increased blood pressure.
In general, AT2 receptors are suggested to oppose the actions mediated by AT1 receptors. Recently, we found that renal AT2 receptor expression is increased and mediates natriuresis in diabetic and obese rat models. Also, we observed that AT2 receptors may have roles in inflammation, lipid metabolism and insulin sensitivity, which interrelated phenomenon in obesity. Our studies also revealed that AT2R may be more beneficial in females in reducing obesity.
We expect that our molecular and whole-animal studies will indicate a therapeutic target to treat obesity and related health disorders, including hypertension, diabetes and kidney disease.
- UH Award for Excellence in Scholarship and Research (Associate Professor Level), University of Houston, 2010
- Teaching Excellence Award, Rho Chi Society (Beta Omicron Chapter), University of Houston College of Pharmacy, 2009, 2004 and 2002
- Honorable Mention - Teaching Excellence Award, Rho Chi Society (Beta Omicron Chapter), University of Houston College of Pharmacy, 2008
- Faculty Service Excellence Award, University of Houston College of Pharmacy, 2006
- Young Scientist Travel Award, Aventis Pharmaceuticals, Renal and Cardiovascular Symposium at Jackson, MS, 2001
- Junior Scientist Award (Physiology), Association of Scientists of Indian Origin in America, Annual Meeting of Experimental Biology, 1994.
- Young Scientist Travel Award, American Society of Pharmacology and Experimental Therapeutics. "Adenosine Receptor Agonist-Induced Regulation of G-proteins in Coronary Artery," 1992
- Cellular Pharmacology (History of cell signaling: receptor, cAMP, IP3, G proteins)
- Cardiovascular/Renal Pharmacology/Physiology (Renin angiotensin system)
- Advanced Pharmacology (Insulin signaling and resistance, treatment of diabetes)
- Cellular Life Science I (proteins/enzymes, carbohydrate metabolism, cell-cycle and cancer)
Nag S, Patel S, Mani S, Hussain T. Role of angiotensin type 2 receptor in improving lipid metabolism and preventing adiposity. Mol Cell Biochem. 2019 Aug 14. doi: 10.1007/s11010-019-03602-y. [Epub ahead of print] PMID: 31414336
Patel S, Dhande I, Gray EA, Ali Q, Hussain T. Prevention of lipopolysaccharide-induced CD11b+ immune cell infiltration in the kidney: role of AT2 receptors. Biosci Rep. 2019 May 23;39(5). pii: BSR20190429. doi: 10.1042/BSR20190429. Print 2019 May 31. PMID: 31072913
Patel S, Hussain T. Dimerization of AT2 and Mas Receptors in Control of Blood Pressure. Curr Hypertens Rep. 2018 May 1;20(5):41. doi: 10.1007/s11906-018-0845-3. Review. Erratum in: Curr Hypertens Rep. 2018 May 12;20(5):47. PMID: 29717388
Patel S, Hussain T. Role of AT2R (Angiotensin Type 2 Receptor) in Maintaining Sodium-Potassium Balance. Hypertension. 2018 Apr;71(4):563-565. doi: 10.1161/HYPERTENSIONAHA.117.10552. Epub 2018 Feb 26. PMID: 29483226
Patel SN, Narayan P, Goldberg ID, Naaz F, Hussain T, Ali Q. Kidney Appreciates Stable Blood Pressure. Am J Hypertens. 2018 Apr 13;31(5):532-533. doi: 10.1093/ajh/hpx212. PMID: 29272328
Patel SN, Ali Q, Samuel P, Steckelings UM, Hussain T. Angiotensin II Type 2 Receptor and Receptor Mas Are Colocalized and Functionally Interdependent in Obese Zucker Rat Kidney. Hypertension. 2017 Oct;70(4):831-838. doi: 10.1161/HYPERTENSIONAHA.117.09679. Epub 2017 Aug 21. PMID: 28827476
Ali Q, Dhande I, Samuel P, Hussain T. Angiotensin type 2 receptor null mice express reduced levels of renal angiotensin converting enzyme-2/angiotensin (1-7)/Mas receptor and exhibit greater high-fat diet-induced kidney injury. J Renin Angiotensin Aldosterone Syst. 2016 Aug 5;17(3). pii: 1470320316661871. doi: 10.1177/1470320316661871. Print 2016 Jul.
Patel S, Ali Q, Hussain T. Angiotensin II type 2-receptor agonist C21 reduces proteinuria and oxidative stress in kidney of high-salt fed obese Zucker rats. Hypertension, 67:906-915, 2016, PMID:25855512
Ali Q, Patel S, Hussain T. Angiotensin AT2 receptor agonist prevents salt-sensitive hypertension in obese Zucker rats. Am. J. Physiol. Renal Physiol 308:F1379-85, 2015
Nag S, Khan MA, Samuel P, Ali Q and Hussain T. Chronic angiotensin AT2R activation prevents high-fat diet-induced adiposity and obesity in female mice independent of estrogen. Metabolism 64:814-25, 2015
Dhande I, Ma W, Hussain T. Angiotensin AT2 receptor stimulation is anti-inflammatory in lipopolysaccharide-activated THP-1 macrophages via increased interleukin-10 production. Hypertens Res 38:21–29, 2015
Hadi NR, Mohammad BI, Alfatlawi H, Qassam HS, Alhar S, Hussain T. Low dose combination of aspirin and clopidogrel retards the progression of atherosclerosis in hypercholesterolemia rabbits via interfering with inflammatory and oxidative responses. American Journal of BioMedicine 3:32-43, 2015
Hadi Hadi NR, Mohammad BI, Ajeena IM, Mahmood A, Majeed SA, Hussain T. Leukotriene synthesis inhibitors modulate atherosclerosis progression in hypercholestrolemic rabbits. Res J Pharmaceut Biol Chem Sci 6(4):362-370, 2014
Ali Q, Nag S, Wu Y, Hussain T. Estimation of angiotensin peptides in biological samples by LC-MS method. Anal Methods 6:215-222, 2014 PMID:24489613
Ali Q, Wu Y, Hussain T. Chronic AT2 receptor activation increases renal ACE2 activity, attenuates AT1 receptor function and blood pressure in obese Zucker rats. Kidney Intl 84:931-939, 2013 PMID:23823602
Dhande I, Ali Q, Hussain T. Proximal tubule angiotensin AT2 receptors mediates an anti-inflammatory response via interleukin-10: Role in renoprotection in obese rats. Hypertension 61:1218-126, 2013, PMID:23547236
Samuel P, Khan MA, Nag S, Inagami T, Hussain T. Angiotensin AT2 receptor contributes towards gender bias in weight gain. PLOS One 8(1):e48425, 2013, PMID:23341867
Samuel P, Ali Q, Sabuhi R, Wu Y, Hussain T. High Na-intake increases renal angiotensin II levels and reduces the expression of ACE2-AT2R-MasR axis in obese Zucker rats. Am J Physiol Renal Physiol 303:F412-419, 2012. PMID:22592638
Ali Q, Hussain T. AT2 receptor non-peptide agonist C21 promotes natriuresis in obese Zucker rats. Hypertens Res, 167:43-47, 2012. PMID:22297475
Asghar M, Tayebati SK, Lokhandwala MF, Hussain T. Potential dopamine-1 receptor stimulation in hypertension management. Curr Hypertens Rep 13:294-302, 2011. Review
Sabuhi R, Ali Q, Asghar M, Al-Zamily NHR, Hussain T. Role of angiotensin II AT2 receptor in inflammation and oxidative stress: Opposing effects in lean and obese Zucker rats. Am J Physiol Renal Physiol 300:F700-F706, 2011. PMID:21209001
Sabuhi R, Asghar M, Hussain T. NADP(H) oxidase inhibition potentiates AT2 receptor agonist induced natriuresis. Am J Physiol Renal Physiol 299(4):F815-20, 2010. PMID:20668101
Ali Q, Sabuhi R, Hussain T. Interferon regulatory factor-1 mediates glucose-induced up-regulation of AT2 receptors in proximal tubules. Mol Cell Biochem, 344(1-2):65-71, 2010. PMID:20596758
Sourris KC, Morley AL, Koita A, Samuel P, Coughlan MT, Thomas MT, Bierhaus A, Naworth PP, Yamamoto H, Allen TJ, Walther T, Hussain T, Cooper ME, Forbes JM. The receptor for advanced glycation end-products (RAGE) exerts renoprotective effects via the AT2 receptor in diabetic nephropathy. Diabetologia 53(11):2442-2451, 2010.
Shahid G, Hussain T. GRK2-deficiency reduces insulin activation of ERK1/2 and mitogenesis in mouse liver FL83B cells. The Open Diabetes Journal 2:44-47, 2009.
Siddiqui AH, Ali Q, Hussain T. Protective role of angiotensin II AT2 receptor in blood pressure increase in obese Zucker rats. Hypertension, 53:256-261, 2009.
Siddiqui AH, Hussain T. Impaired Angiotensin II AT1 receptor function and enhanced Na,K-ATPase affinity for sodium in proximal tubule of streptozotocin-treated rats. Clin Exp Hypertens, 29: 435-444, 2007.
Shahid G, Hussain T. GRK-2 negatively regulates glycogen synthesis in mouse liver FL83B cells. J Biol Chem 242:20612-20620, 2007.
Siddiqui AH, Hussain T. Enhanced AT1 receptor-mediated vasocontractile response to ANG II in endothelium-denuded aorta of obese Zucker rats. Am J Physiol 292:H1722-H1727, 2007.
Hakam AC, Hussain T. Angiotensin II type 2 receptor agonist directly inhibits proximal tubule sodium pump activity in obese but not in lean Zucker rats. Hypertension 47:1117-1124, 2006.
Shah S, Hussain T. Enhanced angiotensin II-induced stimulation of Na,K-ATPase in the proximal tubules of obese Zucker rats. Clin Exp Hypertens. 28:29-40, 2006.
Hakam AC, Hussain T. Renal Angiotensin II AT2 Receptors promote Natriuresis In Streptozotocin-Induced Diabetic Rats. Am J Physiol Renal Physiol. 290:F503-F508, 2006.
Hakam AC, Hussain T. Angiotensin II AT2 receptors Inhibit Proximal Tubular Na+,K+-ATPase Activity via a NO/cGMP Dependent Pathway. Am J Physiol Renal Physiol. 290:F1430-F1436, 2006.
Dhande I, Hussain T. The protective role of angiotensin II type 2 (AT2) receptor in renal disease: Molecular mechanisms and indications for AT2 agonist therapy: The protective arm of renin angiotensin system (RAS) Ed Unger, Thomas, Elsevier 2014
Mustafa, S.J., R. Marala, W. Abebe, N. Jeanssone, H. Olanrewaju, T. Hussain. Coronary adenosine receptor: subtypes, localization and function. In: Adenosine and Adenine Nucleotides, eds. L. Belardinelli and A. Pelleg; pp 229-239, 1994.
Hussain T, Patel SN, Ali Q. Angiotensin II type 2-receptor agonist C21 reduces proteinuria and oxidative stress in kidney of high-salt fed obese Zucker rats. Q. Journal of Hypertension,33(e-Supplement 1):e229, pp.11.17, 2015.
Ali Q, Patel SN, Hussain T. Angiotensin AT2 Receptor Agonist Prevents Salt-Sensitive Hypertension in Obese Zucker Rats. The FASEB J, 2015.
Ali Q, Nag S, Hussain T. AT2 receptor activation prevents high sodium-induced increase in blood pressure and insulin resistance in obese rats. Annual Meeting of the Council on High Blood Pressure Research, 2013.
Dhande I, Hussain T. Angiotensin AT2 Receptor Exerts an Anti-inflammatory response in Lipopolysaccharide-activated THP-1 Macrophages. Annual Meeting of the Council on High Blood Pressure Research, 2013.
Dhande I, Ali Q, Nag S, Khan A, Hussain T. Protective role of the Angiotensin AT2 Receptor in obesity-induced renal inflammation. FASEB J, 2012.
Nag S, Khan MA, Hussain T. Activation of angiotensin type 2 receptor rescues high-fat diet-induced adipocyte size in young male mice. FASEB J, 2012.
Ali Q, Hussain T. Activation of AT2 receptors reduces renal AT1 receptor and enhances ACE2 activity in obese Zucker rats. FASEB J, 2012.
Samuel P, Khan MA, Nag S, Patki G, Lau Y-S, Inagami T, Hussain T. Angiotensin AT2 receptor contributes towards gender bias in weight gain. Presented in High Blood Pressure Research 2011 at Orlando, Fla., September 20-24, pp 8.
Ali Q, Hussain T. Non-peptide Agonist C21 Promotes Natriuresis in Obese rats via Proximal Tubule AT2 Receptor Linked to NO/cGMP Pathway. Presented in High Blood Pressure Research 2011 at Orlando, September 20-24, pp 194.
Ali Q, Samuel P, Sabuhi R, Hussain T. Chronic Treatment with CGP42112A decreases Blood Pressure and lowers Renin Expression in Obese Zucker Rats. American Society Hypertension, 2010.
Samuel P, Ali Q, Sabuhi R and Hussain T. Differential changes in RAS genes and blood pressure by high Na-intake in lean and obese Zucker rats. American Society Hypertension, 2010.
Ali Q and Hussain T. Role of AT2 receptor in long-term blood pressure control in obese Zucker rats. FASEB J, 2009.
Ali Q and Hussain T. Long-term treatment with ATII subtype 2 receptor agonist CGP42112A increases urinary sodium excretion and lowers blood pressure in obese Zucker rats. (April 2009) The Journal of Clinical Hypertension, Supplement A, Volume 11, Issue 4.
Sabuhi R, Siddiqui A and Hussain T. Cross-talk between NAD(P)H oxidase and AT1 receptors in the proximal tubules of obese Zucker rats. FASEB J ,2009.
Hussain T and Suddiqui A.H. Angiotensin II AT1 and AT2 receptors are differentially dysfunctional to regulate Na-K-ATPase (NKA) Activity in the Proximal Tubules of Spontaneously Hypertensive (SHR) rats. FASEB J 22:lb167, 2008.
Hussain T and Siddiqui, A.H. Phospholipase C and NADPH Oxidase Contribute to the Enhanced AT1 receptor mediated Na-K-ATPase Stimulation in the Proximal tubules of Obese Zucker rats. JASN 18:152A, 2007.
Siddiqui, A.H. and Hussain T. Chronic treatment with Angiotensin II type 2 receptor Antagonist Increases Blood Pressure in Obese Zucker Rats. (Presented at the 61st Annual Meeting of Council for High Blood Pressure Research, Westin La Paloma - Tucson, AZ, Sep 26-29, 2007.
Siddiqui, A.H. and Hussain T. Enhanced Na-K-ATPase (NKA) Affinity for Sodium and Reduced AT1 Receptor Function on NKA Activity in the Proximal Tubule of Streptozotocin-Induced Diabetic Rats. FASEB J, 2007.
Hussain T, Fouladi N. Angiotensin II AT2 receptor agonist produces natriuresis in spontaneously hypertensive rats. Hypertension 48:e102, 2006.
Siddiqui A, Hussain T. Na,K-ATPase exhibits higher affinity for sodium and insensitivity to Angiotensin II receptors action in proximal tubule of streptozotocin-induced diabetic rat. Hypertension 48:e101, 2006.
Shahid G, Hussain T. GRK2 differentially regulates insulin-induced glycogen synthesis and mitogenesis. Acta Pharmacologica Sinica Suppl 1:302, 2006.
Hakam A, Hussain T. Role of angiotensin II AT2 receptors in sodium metabolism in obese Zucker rats. Acta Pharmacologica Sinica Suppl 1:414, 2006.
- Member, American Physiological Society
- Member, American Society of Pharmacology and Experimental Therapeutics
- Member, American Society of Nephrology
- Member, American Heart Association
- Member, American Association of Colleges of Pharmacy
- Member, Rho Chi Society
- Associate Editor, Clinical and Experimental Hypertension (2008 – present)
- Editorial Advisory Board Member, The Open Nitric Oxide Journal (2008 – present)
- Editorial Board Member, Clinical and Experimental Hypertension (2002 – 2007)