Molecular Biology

Chapter 10 Outline

RNA polymerases:

∑ Detection and separation of the three RNA polymerases (F10.2, F10.5).

∑ Roles of the three RNA polymerases ≠ which ones transcribe which RNAs (F10.7, T10.1).

∑ RNA polymerase structure (T10.2); epitope tagging (F10.9); requirement and stoichiometry of Pol II subunits (F10.10; T10.3); RNA pol II subunit function for DNA binding (F10.12), transcription initiation at a promoter (F10.13, F10.15), elongation with phosphorylated rbpIIa (F10.17) and alpha amanatin resistance.

Promoters:

∑ Class II core promoters (F10.25).

a) TATA box characteristics; TATA-less promoters and where they are found; TATA box function (F10.26, F10.28, F10.29).

b) Upstream element characteristics; GC boxes and their function (T10.4); CAAT boxes and their function.

c) Initiator sequence and function.

∑ Class I core promoters.

a) Core element and upstream control element (F10.32); spacing among elements (F10.33).

∑ Class III core promoters.

a) Internal promoters and where they are found (F10.34, F10.35); A, B and C boxes and intermediate element (F10.36).

b) Polymerase II-like promoters and where they are found (F10.37).

Enhancers and silencers:

∑ Characteristics of enhancers (10.40).

∑ Characteristics of silencers.